source filifactor alocis 35896 wild type strain atcc  (ATCC)

Journal: Toxins

Article Title: Phylogenetic Analysis of Filifactor alocis Strains Isolated from Several Oral Infections Identified a Novel RTX Toxin, FtxA

doi: 10.3390/toxins12110687

Figure Lengend Snippet: ( A ) Overlapping conserved domains in the FtxA protein sequence, deduced using InterPro. The C-terminal portion displays homology to the Hemolysin-type calcium binding-related domain (IPR010566), and the serralysin-like metalloprotease superfamily (IPR011049), and contains repeats-in-toxins (RTX) calcium-binding nonapeptide repeats (IPR001343), and Hemolysin-type calcium-binding conserved sites (IPR018511). ( B ) One archetypal RTX gene cluster ( hlyCABD ) encodes the E. coli alpha-hemolysin, HlyA. HlyA is synthesized as an inactive protoxin, ProHlyA, which is post-translationally activated in two steps, first via HlyC-directed acylation in the cytoplasm, and then by binding Ca 2+ in the extracellular medium . The cyaE gene of B. pertussis encodes a homologue to E. coli tolC and plays a critical role in the secretion of CyaA . We identified no equivalent to an HlyC-homologue in the F. alocis genomes. The hypothetical protein encoded upstream of ftxA exhibits no apparent homology to hlyC or to other known proteins.

Article Snippet: We here aimed to perform phylogenetic analysis of a genome-sequenced F. alocis type strain (ATCC 35896; CCUG 47790), as well as nine clinical oral strains that we have independently isolated and sequenced, for identification and deeper characterization of novel genomic elements of virulence in this species.

Techniques: Sequencing, Binding Assay, Synthesized

Journal: Toxins

Article Title: Phylogenetic Analysis of Filifactor alocis Strains Isolated from Several Oral Infections Identified a Novel RTX Toxin, FtxA

doi: 10.3390/toxins12110687

Figure Lengend Snippet: Sources of the clinical F. alocis strains, isolated and used in the present work, and their respective ftxA and ldh genotypes.

Article Snippet: We here aimed to perform phylogenetic analysis of a genome-sequenced F. alocis type strain (ATCC 35896; CCUG 47790), as well as nine clinical oral strains that we have independently isolated and sequenced, for identification and deeper characterization of novel genomic elements of virulence in this species.

Techniques: Isolation

Journal: Toxins

Article Title: Phylogenetic Analysis of Filifactor alocis Strains Isolated from Several Oral Infections Identified a Novel RTX Toxin, FtxA

doi: 10.3390/toxins12110687

Figure Lengend Snippet: Phylogenetic relationships among the ten F. alocis strains, based on multilocus sequence typing (MLST) analysis as described in Materials and Methods. The tree with the highest log likelihood is shown. The percentages of trees in which the associated strains clustered together is shown next to the branches. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The ftxA and ldh genotypes, respectively, are indicated.

Article Snippet: We here aimed to perform phylogenetic analysis of a genome-sequenced F. alocis type strain (ATCC 35896; CCUG 47790), as well as nine clinical oral strains that we have independently isolated and sequenced, for identification and deeper characterization of novel genomic elements of virulence in this species.

Techniques: Sequencing

Journal: Toxins

Article Title: Phylogenetic Analysis of Filifactor alocis Strains Isolated from Several Oral Infections Identified a Novel RTX Toxin, FtxA

doi: 10.3390/toxins12110687

Figure Lengend Snippet: Sources of the clinical F. alocis strains, isolated and used in the present work, and their respective ftxA and ldh genotypes.

Article Snippet: We here aimed to perform phylogenetic analysis of a genome-sequenced F. alocis type strain (ATCC 35896; CCUG 47790), as well as nine clinical oral strains that we have independently isolated and sequenced, for identification and deeper characterization of novel genomic elements of virulence in this species.

Techniques: Isolation

Journal: Toxins

Article Title: Phylogenetic Analysis of Filifactor alocis Strains Isolated from Several Oral Infections Identified a Novel RTX Toxin, FtxA

doi: 10.3390/toxins12110687

Figure Lengend Snippet: Phylogenetic relationships among the ten F. alocis strains, based on multilocus sequence typing (MLST) analysis as described in Materials and Methods. The tree with the highest log likelihood is shown. The percentages of trees in which the associated strains clustered together is shown next to the branches. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The ftxA and ldh genotypes, respectively, are indicated.

Article Snippet: We here aimed to perform phylogenetic analysis of a genome-sequenced F. alocis type strain (ATCC 35896; CCUG 47790), as well as nine clinical oral strains that we have independently isolated and sequenced, for identification and deeper characterization of novel genomic elements of virulence in this species.

Techniques: Sequencing

Journal: Molecular oral microbiology

Article Title: Filifactor alocis collagenase can modulate apoptosis of normal oral keratinocytes

doi: 10.1111/omi.12163

Figure Lengend Snippet: Bacterial Strains and Plasmids Used In This Study

Article Snippet: F. alocis cultures were grown anaerobically at 37°C, and maintained in an anaerobic chamber (Coy Manufacturing) with 10% H 2 , 10% CO 2 , and 80% N 2 . table ft1 table-wrap mode="anchored" t5 caption a7 Bacterial Strains Description Source Filifactor alocis ATCC 35896 Wild-type strain Aruni et al ., (2011) Escherichia colI Top 10 Genotype: F- mcrA Δ(mrr- hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacΧ74 recA1 araD139 Δ(ara- leu)7697 galU galK rpsL (StrR) endA1 nupG Life technologies E. coli BL21 StarTM (DE3) Genotype: F- ompT hsdSB (rB- mB-) gal dcm rne131 (DE3) Life technologies pFLL215 PrtFAC (HMPREF0389_00504) in Top 10 E.coli This Study Open in a separate window Bacterial Strains and Plasmids Used In This Study Epithelial Cell Culture Conditions Normal Oral Keratinocytes (NOKs) were obtained as a gift from Dr. Penelope Duerksen- Hughes Laboratory (Division of Biochemistry, Loma Linda University School of Medicine) and grown as previously described ( Piboonniyom et al. , 2003 ).

Techniques: